Identification and antimicrobial resistance of E-coli from clinical samples isolated from patients at Al-Jalaa hospital for trauma and surgery, Benghazi- Libya

Abstract

Escherichia coli (E-coli) is the most extensively studied prokaryotic model organism in biotechnology and microbiology and is a major pathogen of increasing importance due to the rise in antibiotic resistance. Aim: This study was aimed to identify the prevalence and antimicrobial susceptibility pattern of E-coli isolated from patients in AlJalaa hospital for trauma & surgery - Benghazi - Libya. Methods: A total of 108 samples were randomly selected from different wards and outpatient department. The bacterial identification and sensitivity were carried out using BD Phoenix system. Significant bacterial growth on culture of the specimens was processed for identification on the basis of colony morphology, Gram staining, and biochemical reactions. Using the Kirby-Bauer disk diffusion method, the antimicrobial susceptibility test (AST) was conducted. Version 28 of IBM SPSS software was utilized to analyze the data. Results: our results show that E-coli found in 83 patients' sample, E-coli was found to be resistant to majority of antibiotics, whereas sensitive to both Amikacine (43) and Imipenem (36). Conclusion: Our study shows that E-coli found resistant to the majority of antibiotics used in antisensitivity test as it’s a play a major role in infection in hospitals and consider a big health issue. Also, the use of broad-spectrum antibiotic irrationally increases without proper plan, lack of surveillance, suitable antibiotics detection through the period of management and infection control, lead to failure of management process. A further evaluation astudy required to study the prevalence and the resistance of E-coli toward antibiotics.