Antibiogram susceptibility and multidrug Resistance of Staphylococcus aureus Isolated from Al-Jalaa Hospital for Surgery and Accidents - Benghazi - Libya
Keywords:
Antibiogram, Staphylococcus aureus, Hospitalized patientsAbstract
Background: Staphylococcus aureus is a major pathogen of increasing importance due to increasing in antibiotic resistance and a major cause of nosocomial infection. Aim: This study was aimed to identify the prevalence and antimicrobial susceptibility pattern of Staphylococcus aureus bacteria isolated from patients in AL-Jalaa hospital for accidents & surgery - Benghazi - Libya. Methods: A total of 108 samples were randomly selected from different wards and outpatient department. Samples collected was taken from both gender with different age. Specimen isolates was obtained from (urine, swap, tip, Endotracheal tube and blood). The bacterial identification and sensitivity was carried out using BD Phoenix system (BD Diagnostics, Sparks, MD, USA). The descriptive crosssectional study was carried out in the Clinical Microbiology laboratory from January 2023 to April 2023. By inoculation in culture media and aerobic incubation at 37°C were done in accordance with the standard micro-biological procedure. Significant bacterial growth on culture of the specimens was processed for identification on the basis of colony morphology, Gram staining, catalase test and coagulase test. Antimicrobial susceptibility test (AST) was done by the Kirby-Bauer disk diffusion method. IBM SPSS software version 28 was used for data analysis. Chi square test used to determine the difference between each variable in the study P < 0.05 statistically significant. Results: our results shows that staph cocci was resistant to majority of antibiotics , as found resistant to in majority of patients penicillin (104), followed by Ciprofloxacin in (103), then Cefeimpe in (102), Nalidixic acid and Ceftriaxone in (101), Teicarcillin-Clavulanic acid in (100), Nitrofurantoin in (99) Imipenem and Cephalothin in (98), Piperacillin in (96), while a moderate resistance was found in amoxicillin in (56) against this on the other hand found only one antibiotic (Clindamycin) highly sensitive to staph cocci with lower resistance rate. Conclusion: Staphylococci found resistant to the majority of antibiotics used in anti-sensitivity test as it’s a play a major role in infection in hospitals and consider a big health issue. Also the use of broad spectrum antibiotic irrationally increases without proper plan, lack of surveillance, suitable antibiotics detection through the period of management and infection control, lead to failure of management process. A further evaluation and study required to study the resistance of staphylococci toward antibiotics specially Methicillin Resistant Staph aureus
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