Identification and antimicrobial resistance of Pseudomonas aeruginosa isolated from patients at Al-Jala teaching hospital for trauma and surgery, Benghazi, Libya

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen, meaning that it exploits some break in the host defenses to initiate an infection. P aeruginosa infection is a serious problem in patients hospitalized with cancer, cystic fibrosis, and burns. The case fatality rate in these patients is 50 percent. Aim: This study was aimed to identify the prevalence and antimicrobial susceptibility pattern of P aeruginosa isolated from patients in AL-Jala hospital for trauma & surgery - Benghazi - Libya. Methods: A total of 70 samples were randomly selected from different wards and outpatient department. The bacterial identification and sensitivity were carried out using BD Phoenix system. Significant bacterial growth on culture of the specimens was processed for identification on the basis of colony morphology, Gram staining, and biochemical reactions. The Kirby-Bauer disk diffusion method has been employed to perform the antimicrobial susceptibility test (AST). Data analysis was conducted using IBM SPSS software version 28. Results: our results shows that P aeruginosa was distributed with 60% in male patients mostly in ICU with 38.6% and found to be resistant to majority of antibiotics, whereas sensitive to both Polymixin-B (45) and Amikacine (38). Conclusion: Our study shows that, P. aeruginosa found resistant to the majority of antibiotics used in anti-sensitivity test as it’s a play a major role in infection in hospitals and consider a big health issue. Also, the use of broad-spectrum antibiotic irrationally increases without proper plan, lack of surveillance, suitable antibiotics detection through the period of management and infection control, lead to failure of management process. A further evaluation and study required to study the prevalence and the resistance of, P. aeruginosa toward antibiotics.